THYROID

EasyPGX® ready Thyroid cat.no. RT028 (48 test, CE IVD)

 

Main features

Detection of the main mutations of exon 2 (codons 12,13), of exon 3 (codons 61) of the genes KRAS, NRAS, HRAS and of the codons 600 and 601 of the gene BRAF using 8 oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene.

Controls

The kit is provided with the following controls:

• Positive control sample containing a mixture of synthetic DNA sequences that correspond to each mutation detected by this kit in a background of wild-type genomic DNA;
• Negative control.

Sensitivity

The kit allows the detection of low percentages of mutated allele in presence of high amounts of wild-type genomic DNA by real-time amplification
with sequence-specific probes marked with FAM and HEX. LOD down to 0.5%.

Starting material

The kit allows the analysis of DNA extracted from fresh, frozen, formalin-fixed paraffin-embedded (FFPE) tissues, plasma*, and cytological samples.

*Please note that extraction from plasma is sold separately (cat.no. H8040)